Cercetare


Tema

27/10/2019

Microorganismul Paenarthrobacter nicotinovorans (numit anterior Arthrobacter nicotinovorans) este unul din putinele organisme vii ce are capaciåtatea de a descompune alcaloidul nicotina si de a-l utiliza ca sursa de carbon si  nitrogen. Proprietate se datoreaza prezentei mega-plasmidului pAO1, entitate genetica independenta avand dimensiunea de 165 kB. pAO1 a fost complet secventiat si contine 2 grupe mari de gene: un prim grup implicat in metabolismul nicotinei si al doilea grup ce formeaza un operon implicat in metabolismul glucidelor. Este acceptat faptul ca genele implicate in catabolismul nicotinei au fost recrutate prin transfer orizontal de gene si grefate pe un plasmid ancestral, ce continea operonul implicat in metabolismul glucidelor (Igloi &  Brandsch 2003).



Mega-plasmidul pAO1 din Paenarthrobacter nicotinovorans (cu albastru - gene implicate in metabolismul nicotinei, verde - gene implicate in metabolismul glucidelor, rosu - gene implicate in procesele de recombinare, alb - gene cu functie necunoscuta)
















Principala tema de cercetare este organizarea moleculara si biologia megaplasmidul pAO1, in special in legatura cu mecanismele de protectie impotriva stresului oxidativ generat de metabolizarea nicotinei, metabolismul  glucidelor si aplicatiile biotehnologice ale acestor 2 cai. Utilizand o combinatie de tehnici bioinformatice am identificat substrate posibile pentru calea metabolica de degradare a glucidelor codificate de pAO1. Compusii selectati au fost apoi testati si am identificat ca substratul metabolic al celui de-al doilea grup de gene de pe pAO1 este xiloza.


Operonul codificand calea de degradare oxidativa a xilozei codificate de pAO1.


In ultimii ani prioritara a devenit evaluarea aplicatiilor medicale ale derivatilor nicotinici produsi de Paenarthrobacter nicotinovorans. Utilizand metode bioinformatice am testat potentialul derivatilor nicotinici de a se de receptorii nicotinici ai acetilcolinei. Am identificat astfel 6-Hydroxy-Nicotine (6HNic)  ca un potential ligand al receptorilor mentionati. In collaborare cu  dr. Lucian Hritcu am demonstrat ca intradevar 6HNic poate modula activitatea receptorilor nicotinici si imbunatati memoria intr-un model animal al bolii Alzheimer. In prezent, dezvoltam o metoda biotehnologica de producere a 6HNIC.


Cultura de Paenarthrobacter nicotinovorans intr-un microfermentator de 3L.



O cultura de 48 ore vizualizata in 1 min si 30 sec, credit: Lector. Dr. Eugen Ungureanu.

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Finantare

14/10/2016

  • PN-III-P2-2.1-TE-2016-0367 - Developing an Arthrobacter nicotinovorans biotechnology for neuro-pharmaceuticals production

  • PN-III-P2-2.1-PED-2016-0177 - Steps towards an Arthrobacter nicotinovorans biotechnology for neuro-pharmaceuticals production

  • PN-II 50BM/2016 - Romania - P.R.P. China joint research project - Nicotine - from toxic residue to metabolic derivatives with neuroprotective effects

  • PN-II-RU-TE-2014-4-0106 - Effects of 6-hydroxy-nicotine on chlorisondamine-induced oxidative stress and neurotoxicity: relevance for Alzheimer’s disease

  • Proiectul de cercetare GI-2014-02 - Grant de cercetare intern pentru tineri cercetători acordat de către Universitatea “Alexandru Ioan Cuza” din Iaşi

  • Proiectul de cercetare CNCSIS TD-236/2007, Implicatiile moleculare ale megaplasmidului pAO1 in metabolismul speciei Arthrobacter nicotinovorans

  • Proiectul de cercetare UEFISCDI PD-337/2010, Clonarea si caracterizarea cadrelor de lectura ORF32 and ORF40 de pe megaplasmidul pAO1 - potentiale modele de studiu a interactiunilor tagatoza-proteine

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Editor pentru:                                                                                                            2/09/2013


Publicatii

8/12/2019

Articole în ultimii 5 ani

  1. Mihasan, M.; Babii, C.; Aslebagh, R.; Channaveerappa, D.; Dupree, E. & Darie, C. C.,  Proteomics based analysis of the nicotine catabolism in Paenarthrobacter nicotinovorans pAO1, Scientific Reports, 2018, 8, Article number: 16239 [Fulltext]
  2. Ionita R, Postu PA, Mihasan M, Gorgan DL, Hancianu M, Cioanca O, Hritcu L. 2018. Ameliorative effects of Matricaria chamomilla L. hydroalcoholic extract on scopolamine-induced memory impairment in rats: A behavioral and molecular study. Phytomedicine 47:113–120.[Fulltext]
  3. Kelly L. Wormwood, Armand Gatien Ngounou Wetie, Marcus Vinicius Gomez, Yue Ju, Paul Kowalski, Marius Mihasan, Costel C. Darie -  Structural Characterization and Disulfide Assignment of Spider Peptide Phα1β by Mass Spectrometry, Journal of The American Society for Mass Spectrometry,  2018 29(5):827-841 [PubMed]
  4. Craita Maria Rosu, Mihaela Avadanei, Daniela Gherghel, Marius Mihasan, Cosmin Mihai, Adriana Trifan, Anca Miron, Gabriela Vochita - Biodegradation and Detoxification Efficiency of Azo-Dye Reactive Orange 16 by Pichia kudriavzevii CR-Y103, Water, Air, & Soil Pollution,  2018, 229:15  [Fulltext]
  5. Postu, PA, Noumedem, JAK, Cioanca, O, Hancianu, M, Mihasan, M, Ciorpac, M, Gorgan, DL, Petre, BA,  Hritcu, L., Lactuca capensis reverses memory deficits in A beta 1-42-induced an animal model of Alzheimer's disease, Journal of Cellular and Molecular Medicine 22(1):111-122  [PubMed]
  6. Radu, I.; Valu, V. M.; Postu, P. A.; Cioanca, O.; Hritcu, L. & Mihasan, M. 6-Hydroxy-L-Nicotine effects on anxiety and depression in a rad model of chlorizondamine Farmacia, 2017, 65, 237-240 [Fulltext]
  7. Ionita R, Postu PA, Beppe GJ, Mihasan M, Petre BA, Hancianu M, Cioanca O, Hritcu L., Cognitive-enhancing and antioxidant activities of the aqueous extract from Markhamia tomentosa (Benth.) K. Schum. stem bark in a rat model of scopolamine. Behavioral and Brain Functions 2017 13:5 [PubMed]
  8. Hritcu Lucian, Ionita Radu, Motei Diana Elena, Babii Cornelia, Stefan Marius, Mihasan Marius. 2017. “Nicotine versus 6-Hydroxy-L-Nicotine against Chlorisondamine Induced Memory Impairment and Oxidative Stress in the Rat Hippocampus.” Biomedicine & Pharmacotherapy 86: 102–8. [Fulltext]
  9. M Mihăşan, R Brandsch. A predicted T4 secretion system and conserved DNA-repeats identified in a subset of related Arthrobacter plasmids, Microbiological Research, 2016, 191, 32-37  [PubMed]
  10. Caliga, R.; Maniu, C. L. & Mihasan, M. ELF-EMF exposure decreases the peroxidase catalytic efficiency in vitro Open Life Sciences, 2016, 11, 71-77 [Abstract/Free Full Text]
  11. Hritcu L., Hancianu M., Mihasan M., Cioanca O. - Effects of inhaled juniper volatile oil in amyloid beta (1‐42)‐induced anxiety and depression in laboratory rats, Flavour and Fragrance Journal 31 (2), 149-157 [Abstract/Full Text]
  12. Cornelia Babii, Lucian Gabriel Bahrin, Anca‐Narcisa Neagu, Irina Gostin, Marius Mihasan, Lucian Mihail Birsa, Marius Stefan, Antibacterial activity and proposed action mechanism of a new class of synthetic tricyclic flavonoids, Journal of Applied Microbiology, 2016, 120(3):630-7, [PubMed]
  13. Eyup Bagci, Emel Aydin, Marius Mihasan, Calin Maniu, Lucian Hritcu, Anxiolytic and antidepressant‐like effects of Ferulago angulata essential oil in the scopolamine rat model of Alzheimer's disease, Flavour and Fragrance Journal, 2016 31(1):70-80,
    [Abstract/Free Full Text]
  14. Marius Mihasan, Calin Maniu, Lucian Hritcu, Anxiolytic and antidepressant‐like effects of Ferulago angulata essential oil in the scopolamine rat model of Alzheimer's disease, Flavour and Fragrance Journal, 2016 31(1):70-80,
    [Abstract/Free Full Text]
  15. Galba J Beppe, Alain B Dongmo, Harquin S Foyet, Théophile Dimo, Marius Mihasan, Lucian Hritcu, The aqueous extract of Albizia adianthifolia leaves attenuates 6-hydroxydopamine-induced anxiety, depression and oxidative stress in rat amygdala, BMC Complementary and Alternative Medicine, 2015, 15:374, [PubMed Central]
  16. Mihasan M, Bioinformatics-based molecular classification of Arthrobacter plasmids Cellular and Molecular Biology Letters. DOI: 10.1515/cmble-2015-0036, July 2015, [PubMed]
  17. Hritcu L, Bagci, E Aydin, M Mihasan, Antiamnesic and Antioxidants Effects of Ferulago angulata Essential Oil Against Scopolamine-Induced Memory Impairment in Laboratory Rats, Neurochem Res, 2015, 40:1799–1809 [PubMed]
  18. Hritcu, L.; Stefan, M.; Brandsch, R. & Mihasan, M. Enhanced behavioral response by decreasing brain oxidative stress to 6-hydroxy-l-nicotine in Alzheimer's disease rat model. Neuroscience Letters, 2015, 591, 41-47, [PubMed]
  19. Cioanca, O.; Hancianu, M.; Mihasan, M. & Hritcu, L. Anti-acetylcholinesterase and Antioxidant Activities of Inhaled Juniper Oil on Amyloid Beta (1-42)-Induced Oxidative Stress in the Rat Hippocampus, Neurochemical Research, Springer US, 2015, 1-9, [PubMed]
  20. Hritcu, L.; Noumedem, J.; Cioanca, O.; Hancianu, M.; Postu, P. & Mihasan, M. Anxiolytic and antidepressant profile of the methanolic extract of Piper nigrum fruits in beta-amyloid (1-42) rat model of Alzheimer's disease Behavioral and Brain Functions, 2015, 11, 1 [PubMedCentral]
  21. Mihalache, G.; Zamfirache, M.-M.; Mihasan, M.; Ivanov, I.; Stefan, M. & Raus, L. Phosphate-solubilizing bacteria associated with runner bean rhizosphere Archives of Biological Sciences, 2015
    [Abstract/Free Full Text]
  22. Cioanca, O.; Hritcu, L.; Mihasan, M.; Trifan, A. & Hancianu, M. Inhalation of coriander volatile oil increased anxiolytic–antidepressant-like behaviors and decreased oxidative status in beta-amyloid (1–42) rat model of Alzheimer's disease Physiology & Behavior , 2014, 131: 68-74 [PubMed]
  23. Hritcu, L.; Noumedem, J.; Cioanca, O.; Hancianu, M.; Kuete, V. & Mihasan, M. Methanolic Extract of Piper nigrum Fruits Improves Memory Impairment by Decreasing Brain Oxidative Stress in Amyloid Beta(1-42) Rat Model of Alzheimer?s Disease Cellular and Molecular Neurobiology, 2014, 34(3):437-49 [PubMed]

Lista completă de publicații este disponibilă aici

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20/01/2019

Postere/Prezentari în ultimii 5 ani


  1. Mihasan, M. A surprisingly useful soil microorganism: Paenartharobacter nicotinovorans pAO1,IUT Lille, Host Dr. Francois Krier, December 2019, Lille, France  [Photo] [Photo] [Presentation]
  2. Marius Mihăşan, Cornelia Babii, Roshanak Aslebagh, Devika Channaveerappa, Emmalyn Dupree, Costel C. Darie, Proteomics based analysis of the nicotine catabolism in Paenarthrobacter nicotinovorans pAO1, Conferința Natională a SRBBM, Iași, [Photo] [Photo] [Presentation]
  3. M. Mihasan, C. Babii, D. Channaveerappa, R. Aslebagh, E. Dupree, C. Darie - Time-dependent analysis of Paenarthrobacter nicotinovorans pAO1 nicotine-related proteome, 44th FEBS Congress, From Molecules to Living Systems, Krakow, Poland, July 6‐11, 2019, poster USAP-39-007 [Abstract] [Photo] [Photo] [Photo] [Poster]
  4. M. Mihasan, C. Babii, D. Channaveerappa, R. Aslebagh, E. Dupree, C. Darie - Time-dependent analysis of Paenarthrobacter nicotinovorans pAO1 nicotine-related proteome, 67th ASMS Conference on Mass Spectrometry and Allied Topics, June 2-6 2019, Atlanta, Georgia, USA [Photo] [Photo] [Photo] [Poster]
  5. M. Mihasan, C. Babii, D. Channaveerappa, R. Aslebagh, E. Dupree, C. Darie - Proteomic analysis of nicotine response in Paenarthrobacter nicotinovorans pAO1, 43th FEBS Congress, Biochemistry Forever, 7-12 July 2018, Prague, Czech Republic [Photo] [Photo] [Photo] [Poster]
  6. Marius Mihasan, Roderich Brandsch - Molecular evolution and biotechnological applications of Paenarthrobacter nicotinovorans pAO1 megaplasmid, 2nd Romanian Bioinformatics Seminar, April 18-19, Bucharest, Romania,  [Photo]
  7. Marius, M. Paenarthrobacter nicotinovorans pAO1 - surprising applications of a soil bacteria, Microbial Biotechnology Group, Faculty of Natural & Agricultural Sciences, North-West University, Host Olubukola O. Babalola, Mmabatho, South Africa, October 2017
  8. Mihasan, M. Paenarthrobacter nicotinovorans pAO1 a tool for sustainable production of value-added chemicals, Shanghai Jiao Tong University, School of Life Science & Biotechnology, Host Dr. Honghzi Tang, November 2017, Shanghai, China. 2017 [Photo] [Photo] [Photo]
  9. Mihasan, M., Babii, C., Aslebagh, R., Darie C.C. Nicotine-Induced Proteome of Arthrobacter nicotinovorans, The Protein Society's 31st Annual Symposium (international conference),  July 24 - 27, 2017, Montreal, Canada [Photo] [Photo] [Photo]
  10. Mihasan, M., Babii, C., Aslebagh, R., Darie C.C. Nicotine-Induced Proteome of Arthrobacter nicotinovorans, 65th Annual American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 4-8, 2017 [Photo] [Photo] [Photo]
  11. Marius Mihasan, Arthrobacter nicotinovorans as a tool for sustainable production of value-added chemicals, 30th Annual CAMP Technical Meeting, The Inn on the Lake, Canandaigua, NY, May 18, 2017 [Presentation]
  12. Marius Mihasan, Arthrobacter nicotinovorans pAO1- Why do we need its proteome? State Unversity of New York, Department of Biology, Host Dr. Robert Ewy, May 3 2017, Potsdam, NY, USA [Flyer] [Presentation]
  13. Mihasan, M., Babii, C., Aslebagh, R., Darie C.C. Nicotine-Induced Proteome of Arthrobacter nicotinovorans, Experimental Biology Conference,  Chicago, IL, USA, April  22-26, 2017, [Poster] [Photo]
  14. Mihasan, M., Arthrobacter nicotinovorans pAO1 - a tool for sustainable production of value-added chemicals, Biology, Clarkson University, Department of Biology, Host Dr. Kenneth Wallace, April 21, 2017, Potsdam, NY, USA [Photo] [Photo] [Presentation]
  15. Mihasan, M., Arthrobacter nicotinovorans pAO1as a tool to produce neuroactive compounds, Biology Dept., St Lawrence University, Host Dr. Babasola Fateye, April 18, 2017, Canton, NY, US [Presentation]
  16. Mihasan, M., Arthrobacter nicotinovorans pAO1 - Why do we need its proteome? Clarkson University, School of Arts & Sciences, Walsh Seminar Series, Host Dr. Costel C. Darie, April 2017, Potsdam, NY, USA, [Flyer]
  17. Mihasan, M., Arthrobacter nicotinovorans pAO1 - Why do we need its proteome? State Unversity of New York, Department of Chemistry, Host Dr. Fadi Bou-Abdallah, April 2017, Potsdam, NY, USA [Presentation]
  18. Mihasan, M., Arthrobacter nicotinovorans pAO1 - Why do we need its proteome? Clarkson University, Department of Chemistry & Biomolecular Sciences, Host Dr. Costel C. Darie, March 2017, Potsdam, NY, USA
    [Presentation] [Photo] [Photo]
  19. Andreea Andrei, Marius Mihăşan, 2015,  Comparative analysis of the nic-gene cluster within the Arthrobacter genus, 40th FEBS Congress, The Biochemical Basis of Life, Berlin, Germany, July 4-9, FEBS J., 282 issue s1 pp: 188-188 [Abstract] [Poster]
  20. Marius Mihăşan, 2015, The Arthrobacter genus nic-gene clusters share a modular design, The X th International Congress of Geneticists and Breeders Chisinau, 28 June – 1 July, [Presentation]
  21. Andreea Andrei, Marius Mihăşan, 2015, Nic-gene cluster`s modular design within the Arthrobacter genus,  6th Congress of European Microbiologists (FEMS 2015), 7 - 11 June 2015, Maastricht, Olanda, [Poster]
  22. Marius Mihasan, 2015, Molecular classification and comparative analysis of Arthrobacter genus plasmids, 19th Annual International Conference on Research in Computational Molecular Biology (RECOMB) 2015, 12 - 15 Apr 2015, Varsovia, Polonia [Poster on F1000Posters].
  23. Marius Mihasan, 2014, Modular design of the nic-gene cluster within the Arthrobacter genus, The Annual Scientific Meeting of The Faculty of Biology, [Foto] [Foto][Presentation]


Carti și capitole de carte:


  1. Mihăşan, M., Babii, C., Aslebagh, R., Channaveerappa, D., Dupree, E.J., Darie, C.C., 2019. Exploration of Nicotine Metabolism in Paenarthrobacter nicotinovorans pAO1 by Microbial Proteomics, in: Woods, A.G., Darie, C.C. (Eds.), Advancements of Mass Spectrometry in Biomedical Research, Vol. 1140 of Advances in Experimental Medicine and Biology. pp. 515–529.  [Fulltext]

  2. Mihăşan, M., Wormwood, K.L., Sokolowska, I., Roy, U., Woods, A.G., Darie, C.C., 2019. Mass Spectrometry- and Computational Structural Biology-Based Investigation of Proteins and Peptides, in: Woods, A.G., Darie, C.C. (Eds.), Advancements of Mass Spectrometry in Biomedical Research, Vol. 1140 of Advances in Experimental Medicine and Biology. pp. 265–287.  [Fulltext]

  3. Hritcu, L., Mihasan M., 2019. 6-Hydroxy-l-Nicotine and Memory Impairment 6-Hydroxy-l-Nicotine and Memory Impairment, book chapter in Neuroscience of Nicotine Mechanisms and Treatment, 1st Edition, Editor Preedy V, pages 165-172 [Fulltext]

  4. Mihasan Marius -  Megaplasmidul pAO1 - structura si functie, Editura Universitatii ''Alexandru Ioan Cuza'' din Iasi, 2011, carte finantata de catre CNCSIS-UEFISCSU, PN II-RU 337/2010 [carte in format .pdf]

  5. Marius Mihasan, Marius Stefan, Zenovia Olteanu -  Biologie moleculară - metode experimentale, Editura Universitatii ''Alexandru Ioan Cuza'' din Iasi, 2012, carte finantata de catre CNCSIS-UEFISCSU, PN II-RU 337/2010 [.pdf]

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Colaborari

20/07/2017

  • Grupul de Biochimie si Proteomica condus de Dr. Costel C. Darie, Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY, USA - a facut posibila realizarea unui stagiu de cercetare finantat de Comisia Fulbright Romania. Dr. Darie a pus la dispozitie reactivi, laboratorul si instrumente pentru a stabili proteomul bacteriei Arthrobacter nicotinovorans.


Grupul dr. Darie, Aprilie 2017. De la dreapta la stanga Dr. Darie, Kelly W., Roshi A., Devika C., Emma D. si eu in tricolul cu porcul.


  • Dr. Hongzhi Tang (Robert) şi  Dr. Fei Tao, Group for Food & Environmental Micro-Biotechnol, State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University


In vizita la School of Life Sciences & Biotechnology, Shanghai Jiao Tong University.
De la dreapta la stanga Dr. Fei Tao, Hritcu L., Olteanu Z., Stefan M, eu si Dr. Hongzhi Tang.

  • Grupul condus de prof. dr. Roderich Brandsch, Institut für Biochemie und Molekularbiologie este cel in care mega-plasmidul pAO1 a fost secventiat, asa ca ei sunt cei ne care ofera informatii importante  legate de biologia moleculara a acestui mega-plasmid.


Prof. dr. Roderich Brandsch explicand conceptul sau in arta, Septembbrie 2010, Freiburg (de la stanga la dreapta: dr. Marius Mihasan, prof. dr. Roderich Brandsch, dr. Eugen Ungureanu)

  • Toate problemele legate de enzime si cinetica enzimatica sunt discutate cu prof. dr. Vlad Artenie, Universitatea ''Alexandru I Cuza'', Facultatea de Biologie

  • Detaliile practice legate de operatii ce implica manipularea microorganismelor sunt discutate in detaliu cu Conf. habil. dr. Marius Stefan, Universitatea ''Alexandru I Cuza'', Facultatea de Biologie.


Conferenţiar dr. Marius Stefan in laborator, Decembrie 2010, Iasi

  • Problemele legate de redactarea si trimiterea a manuscriselor, receptori neuronali si neurotransmitatori sunt rezolvate impreuna cu conf. dr. Lucian Hritcu

 

Last  man standing - Conferenţiar dr. Lucian Hritcu este ultimul care pleaca de la  EMBO Meeting 2011, Vienna, Austria

  • Multitudinea de probleme adminsitrative, precum si cele legate de chimie sunt dicutate si rezolvate cu ajutorul conf. dr. Zenovia Olteanu


Studenti straini


Jaurès AK Noumedem, doctorand, Departamentul de Biochimie, Facultatea de Stiinte, Universitatea din Dschang, Cameroon, Bursier ''Eugen Ionescu'',  AUF, 2011 Julie Reghem, masterand, DUT “Genie Biologique”, ABB – Lille, bursier Erasmus Placements, 2013 Victor Baumont, Student, DUT “Genie Biologique”, ABB – Lille, bursier Erasmus Placements, 2015






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Legaturi

14/09/2012

Organizatii ce asigura finantarea cercetarii in Romania:

  • Autoritatea Nationala pentru Cercetare Stiintifica: ANCS

  • Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii: UEFISCDI


Soft pentru andocare moleculara si modelarea structurii 3D a proteinelor:

  • mediu complet, flexibil si modular de modelare moleculara si vizualizare - OpenStructure

  • server complet automat pentru modelarea structurii 3D pe baza de omologie  - SWISS-MODEL

  • o modalitate alternativa de a accesa si interactiona cu SWISS-MODEL - DeepView (Swiss Pdb-Viewer)

  • program pentru modelarea structii 3D a proteinelor pe baza de omologie sau modelare comparativa, nu are interfata grafica - MODELLER

  • o interfata grafica pentru Modeller - MINT

  • program pentru vizualizarea, editarea si analiza structurilor moleculelor - UCSF Chimera

  • suita de programe pentru protein engineering, in special pentru mutageneza (site-directed mutagenesis) -  TRITON

  • program de andocare moleculara, in linie de comanda - DOCK 6

  • o suita de instrumente pentru andocare moleculara automatizata  - AutoDock si o interfata grafica gratuita - ADT

  • unul dintre cele mai populare programe de vizualizare a moleculelor - PyMOL 

  • sistem de operare bootabil si autoconfigurabil pentru screening virtual bazat pe AutoDock4/Vina - MOLA

Software bioinformatic si Linux:

  • utilitare pentru genetică microbiană comparativă  Genomics BioTools  - CMGBiotools

  • briceagul elvetian al staiilor de lucru in bioinformatica - Bio-linux

  • proiect dedicat pentru dezvoltarea sistemului de operare Debian in scopul utilzarii in practica medicala si cercetare  Debian Med

  • o colectie de aplicatii open-source pentru cercetatorii din domeniul stiintelor vietii  BioBrew

  • un comentariu interesant despre Open software for biologists

Baze de date si alte legaturi utile:

  • colectie de metode diponibila on-line: Protocol on-line

  • European Bioinformatics Institute: EMBL-EBI

  • Medline at NIH: PubMed

  • Central European Science Publishers: Versita

  • SIB PROTEOMICS: Expasy

  • World Wide Protein Structure Database: wwPDB and its european partner PDBe

  • DNA and protein Sequnce Manipulation Suite accesibil pe site-ul original SMS sau pe acest site SMS

Companii de distributie reactivi si echipamente:

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