Research

Overview

24/01/2018

Paenarthrobacter nicotinovorans (formerly Arthrobacter nicotinovorans, Arthrobacter oxidans) is a coryneform bacteria able to grow on nicotine as sole carbon and nitrogen source due to the presence of the 165 kb megaplasmid pAO1. The megaplasmid was sequenced and contains 2 major gene clusters: one related to nicotine metabolism and a second cluster forming an operon related to sugar catabolism.





The pAO1 catabolic megaplasmid of Paenarthrobacter nicotinovorans (shades of blue - genes related to nicotine uptake and utilization, green - xylose catabolism related genes, red - recombination related genes, white - unknown function)














I am mainly focused on the molecular biology of the pAO1 megaplasmid related to the defense mechanism against nicotine-generated oxidative stress, sugar-catabolism and biotechnological applications.

Using a unique combination of BLAST searches, protein modeling and in-silico docking I identified putative substrates for the pAO1 coded sugar-operon. The selected compounds were experimentally tested and xylose was identified as the physiological substrate.


The operon coding for a oxidative xylose-catabolic pathway on the pAO1 megaplasmid.


In the last couple of years I focused on the possible medical applications of the nicotine-derivatives produced by Paenarthrobacter nicotinovorans. Using in-silico docking experiments, we tested the potential of nicotine and piridine derivatives produces by this bacteria to bind nAchR and identified 6-Hydroxy-Nicotine (6HNic)  as a potential ligand. In collaboration with  dr. Lucian Hritcu we showed that indeed  6HNic is able to modulate the function of nAChRs and sustain spatial memory formation in a rat model of Alzheimer’s disease. Currently we are working on a biotechnological method to produce and isolate 6HNic using a genetically engineered  Paenarthrobacter nicotinovorans strain.


    


Growing Paenarthrobacter nicotinovorans in a 3L micro-fermenter.


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Funding

14/10/2016
  • PN-III-P2-2.1-PED-2016-0177 - Steps towards an Arthrobacter nicotinovorans biotechnology for neuro-pharmaceuticals production

  • PN-II 50BM/2016 - Romania - P.R.P. China joint research project - Nicotine - from toxic residue to metabolic derivatives with neuroprotective effects

  • PN-II-RU-TE-2014-4-0106 – Effects of 6-hydroxy-nicotine on chlorisondamine-induced oxidative stress and neurotoxicity: relevance for Alzheimer’s disease

  • GI-2014-02 – Internal Research grant awarded by the Alexandru I Cuza University of Iaşi

  • TD-236 research grant, Molecular implications of the pAO1 megaplasmid for the Arthrobacter nicotinovorans

  • PD-337 research grant, Cloning and characterization of ORF32 and ORF40 from the pAO1 megaplasmid of Arthrobacter nicotinovorans -potential protein-tagatose interactions models

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Selected publications

Papers

26/07/2018

  1. Kelly L. Wormwood, Armand Gatien Ngounou Wetie, Marcus Vinicius Gomez, Yue Ju, Paul Kowalski, Marius Mihasan, Costel C. Darie -  Structural Characterization and Disulfide Assignment of Spider Peptide Phα1β by Mass Spectrometry, Journal of The American Society for Mass Spectrometry,  2018 29(5):827-841 [PubMed]
  2. Craita Maria Rosu, Mihaela Avadanei, Daniela Gherghel, Marius Mihasan, Cosmin Mihai, Adriana Trifan, Anca Miron, Gabriela Vochita - Biodegradation and Detoxification Efficiency of Azo-Dye Reactive Orange 16 by Pichia kudriavzevii CR-Y103, Water, Air, & Soil Pollution,  2018, 229:15  [Fulltext]
  3. Postu, PA, Noumedem, JAK, Cioanca, O, Hancianu, M, Mihasan, M, Ciorpac, M, Gorgan, DL, Petre, BA,  Hritcu, L., Lactuca capensis reverses memory deficits in A beta 1-42-induced an animal model of Alzheimer's disease, Journal of Cellular and Molecular Medicine 22(1):111-122  [PubMed]
  4. Radu I., Valu V. M., Postu P.A., Cioanca O., Hritcu L. & Mihasan, M. 6-Hydroxy-L-Nicotine effects on anxiety and depression in a rad model of chlorizondamine Farmacia, 2017, 65, 237-240 [Fulltext]
  5. Ionita R, Postu PA, Beppe GJ, Mihasan M, Petre BA, Hancianu M, Cioanca O, Hritcu L., Cognitive-enhancing and antioxidant activities of the aqueous extract from Markhamia tomentosa (Benth.) K. Schum. stem bark in a rat model of scopolamine. Behavioral and Brain Functions 2017 13:5 [PubMed]
  6. Hritcu Lucian, Ionita Radu, Motei Diana Elena, Babii Cornelia, Stefan Marius, Mihasan Marius. 2017. Nicotine versus 6-Hydroxy-L-Nicotine against Chlorisondamine Induced Memory Impairment and Oxidative Stress in the Rat Hippocampus. Biomedicine & Pharmacotherapy 86: 102–8. [Fulltext]
  7. Mihăşan M, Brandsch R. A predicted T4 secretion system and conserved DNA-repeats identified in a subset of related Arthrobacter plasmids, Microbiological Research, 2016, 191, 32-37  [PubMed]
  8. Caliga, R.; Maniu, C. L. & Mihasan, M. ELF-EMF exposure decreases the peroxidase catalytic efficiency in vitro Open Life Sciences, 2016, 11, 71-77 [Abstract/Free Full Text]
  9. Hritcu L., Hancianu M., Mihasan M., Cioanca O. - Effects of inhaled juniper volatile oil in amyloid beta (1‐42)‐induced anxiety and depression in laboratory rats, Flavour and Fragrance Journal 31 (2), 149-157 [Abstract/Full Text]
  10. Cornelia Babii, Lucian Gabriel Bahrin, Anca‐Narcisa Neagu, Irina Gostin, Marius Mihasan, Lucian Mihail Birsa, Marius Stefan, Antibacterial activity and proposed action mechanism of a new class of synthetic tricyclic flavonoids, Journal of Applied Microbiology, 2016, 120(3):630-7, [PubMed]
  11. Eyup Bagci, Emel Aydin, Marius Mihasan, Calin Maniu, Lucian Hritcu, Anxiolytic and antidepressant‐like effects of Ferulago angulata essential oil in the scopolamine rat model of Alzheimer's disease, Flavour and Fragrance Journal, 2016 31(1):70-80,
    [Abstract/Free Full Text]
  12. Galba J Beppe, Alain B Dongmo, Harquin S Foyet, Théophile Dimo, Marius Mihasan, Lucian Hritcu, The aqueous extract of Albizia adianthifolia leaves attenuates 6-hydroxydopamine-induced anxiety, depression and oxidative stress in rat amygdala, BMC Complementary and Alternative Medicine, 2015, 15:374, [PubMed Central]
  13. Mihasan M, Bioinformatics-based molecular classification of Arthrobacter plasmids Cellular and Molecular Biology Letters, 2015 20(4):612-615, [PubMed]
  14. Hritcu L, Bagci, E Aydin, M Mihasan, Antiamnesic and Antioxidants Effects of Ferulago angulata Essential Oil Against Scopolamine-Induced Memory Impairment in Laboratory Rats, Neurochem Res, 2015, 40:1799–1809 [PubMed]
  15. Hritcu, L.; Stefan, M.; Brandsch, R. & Mihasan, M. Enhanced behavioral response by decreasing brain oxidative stress to 6-hydroxy-l-nicotine in Alzheimer's disease rat model. Neuroscience Letters, 2015, 591, 41-47, [PubMed]
  16. Cioanca, O.; Hancianu, M.; Mihasan, M. & Hritcu, L. Anti-acetylcholinesterase and Antioxidant Activities of Inhaled Juniper Oil on Amyloid Beta (1-42)-Induced Oxidative Stress in the Rat Hippocampus, Neurochemical Research, Springer US, 2015, 1-9, [PubMed]
  17. Hritcu, L.; Noumedem, J.; Cioanca, O.; Hancianu, M.; Postu, P. & Mihasan, M. Anxiolytic and antidepressant profile of the methanolic extract of Piper nigrum fruits in beta-amyloid (1-42) rat model of Alzheimer's disease Behavioral and Brain Functions, 2015, 11, 1 [PubMedCentral]
  18. Mihalache, G.; Zamfirache, M.-M.; Mihasan, M.; Ivanov, I.; Stefan, M. & Raus, L. Phosphate-solubilizing bacteria associated with runner bean rhizosphere Archives of Biological Sciences, 2015
    [Abstract/Free Full Text]
  19. Cioanca, O.; Hritcu, L.; Mihasan, M.; Trifan, A. & Hancianu, M., 2014 - Inhalation of coriander volatile oil increased anxiolytic–antidepressant-like behaviors and decreased oxidative status in beta-amyloid (1–42) rat model of Alzheimer's disease Physiology & Behavior, 131: 68-74 [PubMed]
  20. Hritcu, L.; Noumedem, J.; Cioanca, O.; Hancianu, M.; Kuete, V. & Mihasan, M. Methanolic Extract of Piper nigrum Fruits Improves Memory Impairment by Decreasing Brain Oxidative Stress in Amyloid Beta(1-42) Rat Model of Alzheimer?s Disease Cellular and Molecular Neurobiology, 2014, 34(3):437-49 [PubMed]
  21. Mihasan M, Roderich Brandsch, 2013 - pAO1 of Arthrobacter nicotinovorans and the Spread of Catabolic Traits by Horizontal Gene Transfer in Gram-Positive Soil Bacteria, Journal of Molecular Evolution, 007/s00239-013-9576-x, [PubMed]
  22. Mihasan M, 2013 - What in silico molecular docking can do for the bench-biologist, J. Biosci. 37(6):1- 7 [PubMed]
  23. Marius Mihasan, Marius Stefan, Lucian Hritcu, Vlad Artenie, Roderich Brandsch, 2013 - Evidence of a plasmid encoded oxidative xylose catabolic pathway in Arthrobacter nicotinovorans pAO1. Res. Microbiol., 164(1):22-30, [PubMed]
  24. Cioanca, O.; Hritcu, L.; Mihasan, M. & Hancianu, M. Cognitive-enhancing and antioxidant activities of inhaled coriander volatile oil in amyloid ?(1-42) rat model of Alzheimer's disease Physiology & Behavior, 2013, 120, 193-202 [PubMed]
  25. Mihasan, M.; Luminita, C.; Neagu, E.; Stefan, M. & Hritcu, L. In-Silico Identification Of 6-Hydroxy-L-Nicotine As A Novel Neuroprotective Drug Romanian Biotechnological Letters, 2013, 18, 8333-8340 [Abstract/Free Full Text]
  26. Noumedem JA, Mihasan M, Kuiate JR, Stefan M, Cojocaru D, Dzoyem JP, Kuete V., 2013 - In Vitro antibacterial and antibiotic-potentiation activities of four edible plants against multidrug-resistant gram-negative species, BMC Complement Altern Med. 2013 Jul 25;13(1):190. [PubMed]
  27. Hancianu M, Cioanca O, Mihasan M, Hritcu L., 2013 Neuroprotective effects of inhaled lavender oil on scopolamine-induced dementia via anti-oxidative activities in rats., Phytomedicine., 20(5):446-52 [PubMed]
  28. Marius Stefan, Neculai Munteanu, Vasile Stoleru, Marius Mihasan, Lucian Hritcu, 2013 -  Seed inoculation with plant growth promoting rhizobacteria enhances photosynthesis and yield of runner bean (Phaseolus coccineus L.).  Scientia Horticulturae. 151, 22–29, [Abstract/Free Full Text]
  29. Stefan, M.; Munteanu, N.; Stoleru, V. & Mihasan, M. Effects of inoculation with plant growth promoting rhizobacteria on photosynthesis, antioxidant status and yield of runner bean Romanian Biotechnological Letters, 2013, 18, 8132-8143 [Abstract/Free Full Text]
  30. Noumedem, J.; Mihasan, M.; Lacmata, S.; Stefan, M.; Kuiate, J. & Kuete, V. 2013 Antibacterial activities of the methanol extracts of ten Cameroonian vegetables against Gram-negative multidrug-resistant bacteria BMC Complementary and Alternative Medicine, 13, 26  [Abstract/Free Full Text]
  31. Hritcu L, Stefan M, Brandsch R, Mihasan M., 2012 - 6-hydroxy-L: -nicotine from Arthrobacter nicotinovorans sustain spatial memory formation by decreasing brain oxidative stress in rats. J Physiol Biochem. 2012 Jun 20.[Epub ahead of print] [PubMed]
  32. Hritcu L, Ciobica A, Stefan M, Mihasan M, Palamiuc L, Nabeshima T., 2011 - Spatial memory deficits and oxidative stress damage following exposure to lipopolysaccharide in a rodent model of Parkinson's disease. Neurosci Res. 71(1):35-43 [PubMed]
  33. Hritcu L, Foyet HS, Stefan M, Mihasan M, Asongalem AE, Kamtchouing P., 2011 - Neuroprotective effect of the methanolic extract of Hibiscus asper leaves in 6-hydroxydopamine-lesioned rat model of Parkinson's disease. J Ethnopharmacol. 137(1):585-91 [PubMed]
  34. Stefan M, Hritcu L, Mihasan M, Pricop D, Gostin I, Olariu RI, Dunca S, Melnig V., 2011 - Enhanced antibacterial effect of silver nanoparticles obtained by electrochemical synthesis in poly(amide-hydroxyurethane) media. J Mater Sci Mater Med. 22(4):789-96 [PubMed]
  35. Cobzaru Cristina, Ganas Petra, Mihasan M., Schleberger Paula, Brandsch Roderich, 2011 - Homologous gene clusters of nicotine catabolism, including a new [omega]-amidase for [alpha]-ketoglutaramate, in species of three genera of Gram-positive bacteria, Research in Microbiology,  162(3) 285-291 [PubMed]
  36. Mihasan M, 2010 - Basic protein structure prediction for the biologist: a reviev, Arch. Biol. Sci., Belgrade, 62(4):857-871 [Abstract/Free FullText]
  37. Mihasan M, 2010 - In-silico evidence of a pAO1 encoded tagatose-derivate catabolic pathway in Arthrobacter nicotinovorans, Biologia sect. Cellular and Molecular Biology, vol. 65 (5):760-768 [Abstract/Free FullText]
  38. Ganas P, Sachelaru P, Mihasan M, Igloi GL, Brandsch R., 2008 - Two closely related pathways of nicotine catabolism in Arthrobacter nicotinovorans and Nocardioides sp. strain JS614. Arch Microbiol. 189(5):511-7 [PubMed]
  39. Ganas P, Mihasan M, Igloi GL, Brandsch R., 2007, A two-component small multidrug resistance pump functions as a metabolic valve during nicotine catabolism by Arthrobacter nicotinovorans. Microbiology. 153(Pt 5):1546-55. [PubMed]
  40. Mihasan M, Chiribau CB, Friedrich T, Artenie V, Brandsch R., 2007 - An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism. Appl Environ Microbiol. 73(8):2479-85. [PubMed]
  41. Chiribau CB, Mihasan M, Ganas P, Igloi GL, Artenie V, Brandsch R., 2006 - Final steps in the catabolism of nicotine. FEBS J.;273(7):1528-36. [PubMed]

14/09/2018

Posters/Presentations

  1. M. Mihasan, C. Babii, D. Channaveerappa, R. Aslebagh, E. Dupree, C. Darie - Proteomic analysis of nicotine response in Paenarthrobacter nicotinovorans pAO1, 43th FEBS Congress, Biochemistry Forever, 7-12 July 2018, Prague, Czech Republic [Photo] [Photo] [Photo] [Poster]
  2. Marius Mihasan, Roderich Brandsch - Molecular evolution and biotechnological applications of Paenarthrobacter nicotinovorans pAO1 megaplasmid, 2nd Romanian Bioinformatics Seminar, April 18-19, Bucharest, Romania,  [Photo]
  3. Mihasan, M. Paenarthrobacter nicotinovorans pAO1 a tool for sustainable production of value-added chemicals, Shanghai Jiao Tong University, School of Life Science & Biotechnology, Host Dr. Honghzi Tang, November 2017, Shanghai, China. 2017 [Photo] [Photo] [Photo]
  4. Mihasan, M., Babii, C., Aslebagh, R., Darie C.C. Nicotine-Induced Proteome of Arthrobacter nicotinovorans, The Protein Society's 31st Annual Symposium (international conference),  July 24 - 27, 2017, Montreal, Canada [Photo] [Photo] [Photo]
  5. Mihasan, M., Babii, C., Aslebagh, R., Darie C.C. Nicotine-Induced Proteome of Arthrobacter nicotinovorans, 65th Annual American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 4-8, 2017 [Photo] [Photo] [Photo]
  6. Marius Mihasan, Arthrobacter nicotinovorans as a tool for sustainable production of value-added chemicals, 30th Annual CAMP Technical Meeting, The Inn on the Lake, Canandaigua, NY, May 18, 2017 [Presentation]
  7. Marius Mihasan, Arthrobacter nicotinovorans pAO1- Why do we need its proteome? State Unversity of New York, Department of Biology, Host Dr. Robert Ewy, May 3 2017, Potsdam, NY, USA [Flyer] [Presentation]
  8. Mihasan, M., Babii, C., Aslebagh, R., Darie C.C. Nicotine-Induced Proteome of Arthrobacter nicotinovorans, Experimental Biology Conference,  Chicago, IL, USA, April  22-26, 2017, [Poster] [Photo]
  9. Mihasan, M., Arthrobacter nicotinovorans pAO1 - a tool for sustainable production of value-added chemicals, Biology, Clarkson University, Department of Biology, Host Dr. Kenneth Wallace, April 21, 2017, Potsdam, NY, USA [Photo] [Photo] [Presentation]
  10. Mihasan, M., Arthrobacter nicotinovorans pAO1as a tool to produce neuroactive compounds, Biology Dept., St Lawrence University, Host Dr. Babasola Fateye, April 18, 2017, Canton, NY, US [Presentation]
  11. Mihasan, M., Arthrobacter nicotinovorans pAO1 - Why do we need its proteome? Clarkson University, School of Arts & Sciences, Walsh Seminar Series, Host Dr. Costel C. Darie, April 2017, Potsdam, NY, USA, [Flyer]
  12. Mihasan, M., Arthrobacter nicotinovorans pAO1 - Why do we need its proteome? State Unversity of New York, Department of Chemistry, Host Dr. Fadi Bou-Abdallah, April 2017, Potsdam, NY, USA [Presentation]
  13. Mihasan, M., Arthrobacter nicotinovorans pAO1 - Why do we need its proteome? Clarkson University, Department of Chemistry & Biomolecular Sciences, Host Dr. Costel C. Darie, March 2017, Potsdam, NY, USA
    [Presentation] [Photo] [Photo]
  14. Andreea Andrei, Marius Mihăşan, 2015,  Comparative analysis of the nic-gene cluster within the Arthrobacter genus, 40th FEBS Congress, The Biochemical Basis of Life, Berlin, Germany, July 4-9, FEBS J., 282 issue s1 pp: 188-188 [Abstract] [Poster]
  15. Marius Mihăşan, 2015, The Arthrobacter genus nic-gene clusters share a modular design, The X th International Congress of Geneticists and Breeders Chisinau, 28 June – 1 July, [Presentation]
  16. Andreea Andrei, Marius Mihăşan, 2015, Nic-gene cluster`s modular design within the Arthrobacter genus,  6th Congress of European Microbiologists (FEMS 2015), 7 - 11 June 2015, Maastricht, The Netherlands, [Poster]
  17. Marius Mihasan, 2015, Molecular classification and comparative analysis of Arthrobacter genus plasmids,  19th Annual International Conference on Research in Computational Molecular Biology (RECOMB) 2015, 12 - 15 Apr 2015, Warsaw, Poland, [Poster on F1000Posters].
  18. Marius Mihasan, 2014, Modular design of the nic-gene cluster within the Arthrobacter genus, The Annual Scientific Meeting of The Faculty of Biology, [Foto] [Foto] [Presentation] .
  19. Marius Mihasan, Marius Stefan, Lucian Hritcu, Artenie Vlad, Roderich Brandsch, 2013 - New Insights Into Sugar Catabolism Of Arthrobacter nicotinovorans: Oxidative Degradation Of Xylose, 38th FEBS Meeting, St. Petersburg, Russia,  FEBS J., 280 issue s1, pp: 598-598, SW06.W33–26 [Rezumat][Poster][Foto][Foto]
  20. Marius Mihasan, Marius Stefan, Roderich Brandsch, Lucian Hritcu, 2012 - Identification of 6-hydroxi-nicotine as a novel neuroprotectant with antioxidant properties, 4th International Congress and 30th Annual Scientific Session of RSCB, Satu Mare (Romania) and Debrecen (Hungary) [Presentation]
  21. Marius Mihasan, Marius Stefan, Lucian Hritcu, Vlad Artenie, 2011 - Experimental evidence of a xylose-catabolic  pathway on the pAO1 megaplasmid of  Arthrobacter nicotinovorans, 36th FEBS Meeting, Torino, Italy, FEBS J., 278 issue s1: pp: 170-170  [Abstract] [Poster]
  22. Marius Mihăşan, Marius Stefan, Lucian Hritcu, Vlad Artenie, 2011 -  Cloning and purification of a putative repressor protein from Arthrobacter nicotinovorans pAO1, EMBO Meeting, Vienna, Austria [Abstract][Poster][Photo][Photo]
  23. Mihasan M, 2010 - In-silico evidence of a pAO1 encoded tagatose-derivate catabolic pathway in Arthrobacter nicotinovorans - 35th FEBS Meeting, Gothenburg, Sweden, FEBS J., 277 issue s1: p.248 – 248 [Abstract]
  24. Marius Mihăşan, Vlad Artenie, Zenovia Olteanu, 2010 – In-silico identification of key residues for shifting the coenzyme specificity of an aldehyde-dehidrogenase, Molecular interactions of the living world Science Meeting, Iasi, Romania [Presentation]
  25. Mihasan M, Artenie V, Brandsch R , 2009 - Characterization of a putative carbohydrate catabolic pathway of the megaplasmid pAO1 of Arthrobacter nicotinovorans - FEBS J., 276 suppl. 1: p.113-113 [Abstract] [Poster on eposters.net][Photo]
  26. Mihasan Marius, Vlad Artenie, Roderich Brandsch, 2008 A monomeric aldehyde dehydrogenase as part of an pAO1 encoded pathway for sugar metabolism, FEBS Journal 275 (Suppl. 1), pg. 285 [Abstract] [Poster on eposters.net]

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Colaborations

20/07/2017

  • Dr. Costel C. Darie Biochemistry and Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY, USA - kindly accepted me as a Fulbright Fellow for 6 month and provided lab space, reagents, instruments for solving the nicotine induced proteome of Arthrobacter nicotinovorans


Dr. Darie's group after an department, spring 2017. From left to right Dr. Darie, Kelly W., Roshi A., Devika C., Emma D. and Mr. Mihasan wearing the pig T-shirt.  




  •   Dr. Hongzhi Tang (Robert) and  Dr. Fei Tao, Group for Food & Environmental Micro-Biotechnol, State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University


Visiting the School of Life Sciences & Biotechnology, Shanghai Jiao Tong University.
From left to right, Dr. Fei Tao, Hritcu L., Olteanu Z., Stefan M, me, and Dr. Hongzhi Tang on the extreme right.

  • The group of prof. dr. Roderich Brandsch, Institut für Biochemie und Molekularbiologie kindly provided the Arthrobacter nicotinovorans pAO1+ and pAO1- strains, as well as various other E.coli strains, plasmids and reagents. The pAO1 megaplamid was sequenced by this group, so they also provided valuable information regarding the molecular biology of this catabolic plasmid.



Prof. dr. Roderich Brandsch explaining his art concept, September 2010, Freiburg (from left to right: dr. Marius Mihasan, prof. dr. Roderich Brandsch, dr. Eugen Ungureanu) 


  • All enzymes assays, enzyme kinetics and  sugar biochemistry problems are solved with the help of prof. dr. Vlad Artenie, Alexandru I Cuza University, Faculty of Biology 


  • Most of the microbiology related details and practical work involved in this project are closely discussed with associate professor habil. dr. Marius Stefan, Alexandru I Cuza University, Faculty of Biology.



Marius Stefan performing a CASP-3 assay, December 2010, Iasi.


  • Most of the problems dealing with manuscript writing ans submitting, neuroreceptors and transmitters are solved with the help of associate professor habil.  dr. Lucian Hritcu



Last  man standing - dr. Lucian Hritcu the last to leave the Auditorium at the EMBO Meeting 2011, Vienna, Austria


  • All bunch of adminstrative matters, as well as basic chemistry problems are closely discussed with associate professor dr. Zenovia Olteanu

Visiting students



Jaurès AK Noumedem, PhD student, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon, AUF ''Eugen Ionescu'' scholarship, 2011 Julie Reghem, master student, DUT “Genie Biologique”, ABB – Lille, Erasmus Placements, 2013 Victor Baumont, Bsc student, DUT “Genie Biologique”, ABB – Lille, Erasmus Placements, 2015


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Links

16/12/2014

Funding organisations for romanian universities and postgraduate research programmes:


  • The National Authority for Scientific Research: ANCS

  • Executive Unit for Financing Higher Education, Research, Development and Innovation: UEFISCDI


Protein homology and molecular docking software:

  • Comparative Microbial Genomics BioTools  - CMGBiotools

  • open-source, modular, flexible, molecular modelling and visualization environment - OpenStructure

  • A fully automated protein structure homology-modeling server and model validation  - SWISS-MODEL

  • An alternative way to access and interact with SWISS-MODEL - DeepView (Swiss Pdb-Viewer)

  • Homology or comparative modeling of protein three-dimensional structures, runs locally only via command line (no GUI) - MODELLER

  • A graphical user interface to Modeller program - MINT

  • Automatted homology modelling, flexible protein-protein docking and model validation BISKIT

  • A extensible program for interactive visualization, editing and analysis of molecular structures  - UCSF Chimera

  • Graphical tool for computational protein engineering, mainly site-directed mutagenesis -  TRITON

  • Docking program and tools that must be run localy in a command line DOCK 6

  • Suite of automated docking tools - AutoDock and its free GUI - ADT

  • The new and significantly improved in the average accuracy of the binding mode predictions as well as speed AutoDock Vina

  • One of the most popular programs for molecular visualization - PyMOL with impressive extensibility using various plug-ins:
            performing and visualising docking results with AutoDock/Vina,
            visualization of substrate pathways and entrance tunnels in protein structures CAVER
            visualization of pockets and voids in protein structures detected by CASTp
            create animations and movies with molecular structures - eMovie

  • Bootable, self-configuring system for virtual screening using AutoDock4/Vina - MOLA

Bioinformatics software and Linux:

  • Comparative Microbial Genomics BioTools  - CMGBiotools

  • The swiss army of bioinformatics workstations - Bio-linux

  • A project that aims to develop Debian into an operating system well fit for the requirements for medical practice and biomedical research Debian Med

  • collection of open-source applications for life scientists and an in-house project at Bioinformatics.Org BioBrew

  • A nice commentary on Open software for biologists

Databases and other usefull links:

  • On-line methods collection: Protocol on-line

  • European Bioinformatics Institute: EMBL-EBI

  • Medline at NIH: PubMed

  • Central European Science Publishers: Versita

  • SIB PROTEOMICS: Expasy

  • World Wide Protein Structure Database: wwPDB and its european partner PDBe

  • DNA and protein Sequnce Manipulation Suite: on its original website SMS or mirored by this website SMS2

Life sciences related comercial companies:

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    Contact: marius.mihasan@uaic.ro

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